packaging cell

( 3 ) Introduce the recombinant DNA into retroviral packaging cell line .

重组质粒导入包装细胞；

Establishment of a Packaging Cell Line for Lentivirus Vector

慢病毒载体包装细胞系的建立

Treatment of Rat Hepatoma by Locally Injection of Murine IL-12 Retro-virus Packaging Cell

局部注射小鼠白细胞介素12逆转录病毒包装细胞对大鼠肝癌的治疗

Construction of MAR-mediated retroviral packaging cell line

MAR介导的逆转录病毒包装细胞系的建立

Experimental study of the anti-tumor effects of directly administered packaging cell line of retroviral vector carrying IL-12 gene on hepatoma

携带白细胞介素12基因逆转录病毒载体包装细胞株体内直接注射治疗肝癌的实验研究

Construction of retrovirus expressing CTGF siRNA and its steady packaging cell line mediated by PRS retrovirus vector

PRS-CTGF-SiRNA逆转录病毒载体及包装细胞株的构建

Construction of the recombinant anti-hepatocarcinoma sFv-TNF - α gene retroviral vector and establishment of a stable virus producing packaging cell line

抗肝癌sFv-TNF-α基因逆转录病毒包装细胞系的建立

Objective To observe the transfer and expression of HBV antisense genes and their anti HBV effects in 2.2.15 cells mediated by retroviral vector packaging cell line system .

目的观察重组逆转录病毒载体－包装细胞系统介导反义基因转移表达和抗乙型肝炎病毒（HBV）的作用。

Recombinant adenovirus plasmids were transfected into packaging cell line 293 to generate adenovirus , which were amplified and then purified further .

将腺病毒质粒转染293细胞，包装出重组腺病毒Ad-SP-PR39，并扩增和纯化。

Objective : To evaluate the feasibility and efficacy of combination gene therapy for hepatocellular carcinoma using retroviral packaging cell line encoding interleukin 12 and interleukin 2 genes in rat models .

目的：研究携带小鼠白细胞介素12（mIL-12）和人白细胞介素2（hIL-2）基因的逆转录病毒包装细胞株对大鼠肝癌进行联合基因治疗的可行性和疗效。

Anti-HBV effects of genetically engineered replication-defective HBV with combined expression of antisense RNA and dominant negative mutants of core protein and construction of first-generation packaging cell line for HBV vector

基因重组HBV联合表达反义RNA和显性阴性突变体抗HBV作用及HBV包装细胞系的构建

The anti-tumor effects of the mf ⅶ - SEA chimeric protein were evaluated . Methods Fusion of SEA and mf ⅶ cDNA was constructed using adenovirus vector and produced in 293 packaging cell lines .

方法构建表达Ⅶ因子SEA融合蛋白的腺病毒载体，用293包装细胞系制备病毒Ad/mfⅦSEA。

Conclusion ; The retroviral packaging cell line encoding mIL-12 and hIL-2 gene via intratumoral injection inhibits the growth of hepatocellular carcinoma significantly . The therapeutical effects of early administration is superior to that of later one .

结论：肝癌局部注射携带mIL-12和hIL-2基因的逆转录包装细胞株可明显抑制肝癌细胞的生长，早期治疗优于晚期治疗。

This paper mainly describes the study work on the new approaches of biotherapy for cancer during the period of The 9th Five_year Program for scientific study in China . Construction of recombinant adenoviral vector containing human TNF , IL_2 and B7 genes and establishment of its packaging cell line ;

本文简述九五攻关期间肿瘤生物治疗新方法的研究课题组经协作攻关主要完成的研究工作有：重组人TNF、IL2及B7重组腺病毒表达载体的构建及其包装体系的建立；